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Therefore, a total of twelve different formula combinations of semaglutide and cagrilintide are obtained in the dual-chamber syringe: Semaglutide without histidine, with each cagrilintide formula with glutamate (four different concentrations) Semaglutide with 10 mM histidine, with each cagrilintide formula with glutamate (four different concentrations) Semaglutide with 10 mM histidine, with each cagrilintide formula with lactate (four different concentrations)

The supernatant serum was carefully aspirated using sterile pipettes to avoid disturbing the clot and cellular components, then aliquoted into labeled cryovials (0.5 mL per aliquot to avoid repeated freezethaw cycles), and stored at 20 C until biochemical analysis

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( Epub 2013/09/07 )